Current Development in Oceanography
Volume 1, Issue 2, Pages 69 - 83
(August 2010)
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DETERMINATION OF TETRODOTOXINS (TTXs) AND PARALYTIC SHELLFISH POISONING (PSP) TOXINS IN PUFFER FISH FROM MALAYSIA AND JAPAN BY APPLICATION OF A ZIC-HILIC COLUMN AND MASS SPECTROMETRIC DETECTION
T. Helbig and B. Luckas
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Abstract:
Paralytic shellfish poisoning (PSP) toxins are produced by marine dinoflagellates and some cyanobacteria, whereas tetrodotoxins (TTXs) formed mainly by bacteria are widespread in marine and terrestrial animals, especially in puffer fish. Moreover, both groups of toxins were detected in puffer fish.
PSP toxins and TTXs act as potent blockers of sodium channels. Therefore, positive results obtained by application of the mouse bioassay, the official method for the determination of PSP toxins and TTXs, have to be confirmed with regard to the causative toxin group.
The newly developed method for the simultaneous determination of PSP toxins and TTXs described below is based on the application of hydrophilic interaction liquid chromatography (HILIC) followed by mass spectrometric detection. The chromatographic separation of the PSP toxins and TTXs in one chromatographic run was achieved using a zwitterionic (ZIC-) HILIC column.
By the application of this method, puffer fish of different provenience was analyzed. TTXs were determined in separated tissues of marine puffer fish Lagocephalus wheelerie from Malaysia and as well in different puffer fish from Japan that also contained PSP toxins in lower concentrations. |
| Keywords and phrases: PSP toxins, tetrodotoxins, puffer fish, LC-MS/MS, hydrophilic interaction liquid chromatography. |
| Communicated by Hyo Choi |
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