Cucumber (Cucumis sativus L.), is one of the most important and broadly used vegetables in the world. Ultrasound is widely used in medicine and biotechnology. At the right frequency and exposure period, these waves have desired effects. In the present study, 2-week-old plants, obtained from the seeds of cucumber, were put in an ultrasonic bath with a nominal frequency of 40 kHz, a central frequency of 34/722 kHz and a bandwidth of 320 Hz for 0, 5, 10 and 15 minutes. Then, hypocotyl pieces were used as explants. To obtain appropriate conditions for callus formation, the explants were placed in Murashige and Skoog medium­­ supplemented with 0.5 mg/l each of 2,4-dichlorophenoxyacetic acid, 1-Naphthalene acetic acid and 6-Benzylaminopurine. The mature somatic embryos at the torpedo stage were isolated and encapsulated using sodium alginate to produce artificial seeds. The seeds were transferred to the greenhouse and turned into plants. The use of ultrasonication at a nominal frequency of 40 kHz for 10 minutes showed better results as compared with the samples treated for 0, 5 and 15 minutes, in terms of the shape, maturity of the embryos, and also the germination of artificial seeds and maturing to flowering stages. Since the cucumber variety Dastgerd Esfahan is famous for its aroma and taste and it is somewhat at risk of extinction, we studied the production of artificial seeds of this plant. According to the literature review, there is no study on the effects of ultrasound on embryogenesis and artificial seed production in cucumber.

ultrasound, cucumber, somatic embryogenesis, artificial seed.

Received: February 4, 2020; Accepted: March 15, 2021; Published: June 2, 2021

How to cite this article: Morvarid Koochani, Ahmad Majd, Sedigheh Arbabian, Faezeh Ghanati and Sayeh Jafari Marandi, Effects of ultrasound on somatic embryogenesis and artificial seed production in cucumber (Cucumis sativus L.), JP Journal of Biostatistics 18(2) (2021), 249-267. DOI: 10.17654/BS018020249

This Open Access Article is Licensed under Creative Commons Attribution 4.0 International License

Reference:

[1] S. Bose, J. Karmakar, D. P. Fulzele, U. Basu and T. K. Bandyopadhyay, In vitro shoots from root explant, their encapsulation, storage, plant recovery and genetic fidelity assessment of Limonium hybrid ‘Misty Blue’: a florist plant, Plant Cell Tissue Organ Cult. 129(2) (2017), 313-324.
[2] P. P. Chee and D. M. Tricoli, Somatic embryogenesis and plant regeneration from cell suspension cultures of Cucumis sativus L, Plant Cell Rep. 7(4) (1988), 274-277.
[3] B. Chen, J. Huang, J. Wang and L. Huang, Ultrasound effects on the antioxidative defense systems of Porphyridium cruentum, Colloids Surf B Biointerfaces 61(1) (2008), 88-92.
[4] M. K. Cheruvathur, G. K. Kumar and T. D. Thomas, Somatic embryogenesis and synthetic seed production in Rhinacanthus nasutus (L.) Kurz, Plant Cell Tissue Organ Cult. 113(1) (2013), 63-71.
[5] J. A. T. Da Silva and J. Dobránszki, Sonication and ultrasound: impact on plant growth and development, Plant Cell Tissue Organ Cult. 117(2) (2014), 131-143.
[6] D. Das, A. Rahman, D. Kumari and N. Kumari, Synthetic seed preparation, germination and plantlet regeneration of Litchi (Litchi chinensis Sonn.), Am. J. Plant Sci. 7(10) (2016), 1395.
[7] I. Debeaujon and M. Branchard, Somatic embryogenesis in Cucurbitaceae, Plant cell, tissue and organ culture 34(1) (1993), 91-100.
[8] D. B. Duncan, Multiple range and multiple F tests, Biometrics 11(1) (1955), 1-42.
[9] K. M. S. Elmeer and M. J. Hennerty, Observations on the combined effects of light, NAA and 2, 4-D on somatic embryogenesis of cucumber (Cucumis sativus) hybrids, Plant Cell Tissue Organ Cult. 95(3) (2008), 381-384.
[10] S. Gantait, S. Kundu, N. Ali and N. C. Sahu, Synthetic seed production of medicinal plants: a review on influence of explants, encapsulation agent and matrix, Acta Physiol. Plant. 37(5) (2015), 98.
[11] H. Gawronska, W. Burza, E. Bolesta and S. Malepszy, Zygotic and somatic embryos of cucumber (Cucumis sativus L.) substantially differ in their levels of abscisic acid, Plant Sci. 157(1) (2000), 129-137.
[12] F. Ghanati, M. Safari and A. Hajnorouzi, Partial clarification of signaling pathway of taxanes increase biosynthesis by low intensity ultrasound treatment in hazel (Corylus avellana) cells, S. Afr. J. Bot. 96 (2015), 65-70.
[13] C. Gopi and P. Ponmurugan, Somatic embryogenesis and plant regeneration from leaf callus of Ocimum basilicum L, J. Biotechnol 126(2) (2006), 260-264.
[14] S. M. Haque and B. Ghosh, High-frequency somatic embryogenesis and artificial seeds for mass production of true-to-type plants in Ledebouria revoluta: an important cardioprotective plant, Plant Cell Tissue Organ Cult. 127(1) (2016), 71-83.
[15] S. M. Haque and B. Ghosh, Regeneration of Cytologically Stable Plants Through Dedifferentiation, Redifferentiation, and Artificial Seeds in Spathoglottis plicata Blume, (Orchidaceae), Horticultural Plant Journal 3(5) (2017), 199-208.
[16] A. M. Hassanein, Somatic embryogenesis of cucumber (Cucumis sativus L) using seed cuttings obtained from pre-mature fruit, Plant Biotechnol (Tsukuba) 20(4) (2003), 275-281.
[17] J. Hou, Y. Wu, Y. Shen, Y. Mao, W. Liu, W. Zhao and L. Wu, Plant regeneration through somatic embryogenesis and shoot organogenesis from immature zygotic embryos of Sapium sebiferum Roxb, Sci. Hortic. 197 (2015), 218-225.
[18] T. Isah, Adjustments to in vitro culture conditions and associated anomalies in plants, Acta Biol. Cracov. Bot. 57(2) (2015), 9-28.
[19] H.-J. Ju, J. Jeyakumar, M. Kamaraj, N. Praveen, I.-M. Chung, S.-H. Kim and M. Thiruvengadam, High frequency somatic embryogenesis and plant regeneration from hypocotyl and leaf explants of gherkin (Cucumis anguria L.), Sci. Hortic. 169 (2014), 161-168.
[20] V. N. Juturu, G. K. Mekala and P. Kirti, Current status of tissue culture and genetic transformation research in cotton (Gossypium spp.), Plant Cell Tissue Organ Cult. 120(3) (2015), 813-839.
[21] H. G. A. Kumar, H. N. Murthy and K. Y. Paek, Embryogenesis and plant regeneration from anther cultures of Cucumis sativus L, Sci. Hortic. 98(3) (2003), 213-222.
[22] Y. Liu, A. Yoshikoshi, B. Wang and A. Sakanishi, Influence of ultrasonic stimulation on the growth and proliferation of Oryza sativa Nipponbare callus cells, Colloids Surf B Biointerfaces 27(4) (2003), 287-293.
[23] H. Lou and S. Kako, Role of high sugar concentrations in inducing somatic embryogenesis from cucumber cotyledons, Sci. Hortic. 64(1-2) (1995), 11-20.
[24] T. Murashige and F. Skoog, A revised medium for rapid growth and bio assays with tobacco tissue cultures, Physiol Plant 15(3) (1962), 473-497.
[25] M. Nowacka and M. Wedzik, Effect of ultrasound treatment on microstructure, colour and carotenoid content in fresh and dried carrot tissue, Appl. Acoust. 103 (2016), 163-171.
[26] N. Passalacqua, P. Guarrera and G. De Fine, Contribution to the knowledge of the folk plant medicine in Calabria region (Southern Italy), Fitoterapia 78(1) (2007), 52-68.
[27] P. K. Pati, S. P. Rath, M. Sharma, A. Sood and P. S. Ahuja, In vitro propagation of rose-a review, Biotechnol. Adv. 24(1) (2006), 94-114.
[28] M. K. Rai, P. Asthana, S. K. Singh, V. Jaiswal and U. Jaiswal, The encapsulation technology in fruit plants-a review, Biotechnol. Adv. 27(6) (2009), 671-679.
[29] K. Rajewska and D. Mierzwa, Influence of ultrasound on the microstructure of plant tissue, Innov. Food Sci. Emerg. Technol. 43 (2017), 117-129.
[30] C. S. Raju, K. Kathiravan, A. Aslam and A. Shajahan, An efficient regeneration system via somatic embryogenesis in mango ginger (Curcuma amada Roxb.), Plant Cell Tissue Organ Cult. 112(3) (2013), 387-393.
[31] K. Redenbaugh, B. D. Paasch, J. W. Nichol, M. E. Kossler, P. R. Viss and K. A. Walker, Somatic seeds: encapsulation of asexual plant embryos, Nat. Biotechnol. 4(9) (1986), 797.
[32] A. Rezaei, F. Ghanati, M. Behmanesh and M. Mokhtari-Dizaji, Ultrasound-potentiated salicylic acid-induced physiological effects and production of taxol in hazelnut (Corylus avellana L.) cell culture, Ultrasound Med. Biol. 37(11) (2011), 1938-1947.
[33] M. Safari, F. Ghanati, M. Behmanesh, A. Hajnorouzi, B. Nahidian and M. Ghahremani, Enhancement of antioxidant enzymes activity and expression of CAT and PAL genes in hazel (Corylus avellana L.) cells in response to low-intensity ultrasound, Acta. Physiol. Plant. 35(9) (2013), 2847-2855.
[34] A. Sharififar, M. Nazari and H. R. Asghari, Effect of ultrasonic waves on seed germination of Atriplex lentiformis, Cuminum cyminum, and Zygophyllum eurypterum, J. Appl. Res. Med. Aromat Plants 2(3) (2015), 102-104.
[35] S. Sharma and A. Shahzad, Encapsulation technology for short-term storage and conservation of a woody climber, Decalepis hamiltonii Wight and Arn, Plant Cell Tissue Organ Cult. 111(2) (2012), 191-198.
[36] A. Sunandar and E. D. J. Supena, Induction of Somatic Embryogenesis in Sengon (Falcataria moluccana) With Thidiazuron and Light Treatments, Hayati J. Biosci. 24(2) (2017), 105-108.
[37] T. A. Thorpe, History of plant tissue culture, Mol. Biotechnol. 37(2) (2007), 169-180.
[38] I. K. Vasil, A history of plant biotechnology: from the cell theory of Schleiden and Schwann to biotech crops, Plant Cell Rep. 27(9) (2008), 1423.
[39] M. Y. Vdovitchenko and I. N. Kuzovkina, Artificial seeds as a way to produce ecologically clean herbal remedies and to preserve endangered plant species, Moscow Univ. Biol. Sci. Bull. 66(2) (2011), 48.
[40] S. Von Arnold, I. Sabala, P. Bozhkov, J. Dyachok and L. Filonova, Developmental pathways of somatic embryogenesis, Plant Cell Tissue Organ Cult. 69(3) (2002), 233-249.
[41] H. Yang, J. Gao, A. Yang and H. Chen, The ultrasound-treated soybean seeds improve edibility and nutritional quality of soybean sprouts, Food Res. Int. 77 (2015), 704-710.